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Integral World: Exploring Theories of Everything
An independent forum for a critical discussion of the integral philosophy of Ken Wilber
Frank Visser, graduated as a psychologist of culture and religion, founded IntegralWorld in 1997. He worked as production manager for various publishing houses and as service manager for various internet companies and lives in Amsterdam. Books: Ken Wilber: Thought as Passion (SUNY, 2003), and The Corona Conspiracy: Combatting Disinformation about the Coronavirus (Kindle, 2020).
TABLE OF CONTENTS | REVIEWS
"The peer reviews [of the Corman-Drosten Review Report] overall come to the same conclusion as experts who have published their 'reviews' of the retraction request (see some examples: Beyer 1, Beyer 2, Visser, Wilson)." Andrea Ammon, Director European Centre for Disease Prevention and Control, www.asktheeu.org, 2021-06-14.
|Taxonomic classification of the SARS-CoV-2 virus|
Severe acute respiratory
So just remember, SARS-CoV-2 belongs indeed to the very large family of the coronaviruses, but has nothing to do with the common cold. Different genus, different subgenus, different species, different strain. The Corman-Drosten test specifically aims at the SARS-related coronavirus species, catching several of its strains.
This is basic virological knowledge which even Wikipedia can teach you, but which is lost on conspiracy-minded pseudo-scientists like McKernan and Mahlhotra (not to mention sensationalist authors/journalists who feed on them like Naomi Wolf).
TIME TO LET CHRISTIAN DROSTEN HAVE HIS SAY...
It is unfortunatethough perhaps understandablethat none of the Corman-Drosten paper authors has dealt with the Review Report points of criticismthat I must grant to their disgruntled authors. Obviously, the tone of voice of the Review Report didn't invite any such discussion and possibly alienated parts of their potential audience (though with an alleged 20 million page views, that audience has been extremely large for a "scientific" paper). Yet Christian Drosten has appeard in German media frequently in the past twelve months, and in this March 2020 fragment from the Norddeutscher Rundfunk radio he discusses similar concerns about his PCR test:
Korinna Hennig: The critical voices, in particular the quoted Wolfgang Wodarg, also relate directly to the PCR test, i.e. the genetic test for the virus that you and your team have developed and that is used worldwide. It has not been validated at all, i.e. has been adequately scientifically assessed, and has not been created at all on the basis of the current SARS-CoV-2 genome and would therefore also display everything else possible. Can you briefly explain to us how you established the PCR test back then and who was looking at it, in layman's terms?
Christian Drosten: This test was done at a time when we didn't even have this virus in the country or in the laboratory. And we have started developing it based on the closely related SARS coronavirus. But this has nothing to do with the common cold coronaviruses. Genetically, that's very far. And we did two tests. In fact, we actually did a whole series of testscandidate tests. And they were made on the basis of the old SARS coronavirus and a huge diversity of bat coronaviruses, i.e. the closest relatives that are all in the same virus type. And we've done tests that would detect a number of these coronaviruses. So these animal corona viruses, bat corona viruses, would also be recorded. And the old SARS coronavirus and then with some probability the new one too. And then the sequence of the new coronavirus came out. Then we matched that.
Korinna Hennig: From China.
Christian Drosten: From China, from colleagues, but only theoretically as sequence information. Then we took the two tests from our candidate tests that are particularly good for this new virus. We then further validated them, with the University of Hong Kong, the University of Rotterdam, the national public health organization in London and our own patients. A very, very large validation study has been done. I would have to open it on my computer now to go back to the numbers. But we have large numbers of real patient sampleswith known positive evidence of other coronaviruses and also every other cold virus we know, and a whole number of them for each individual virusa whole number of patient samples, that is, hundreds of samples with other coronaviruses and other cold virus, we tested in this test. And not once has there been a false positive reaction. So this test does not react against any other human coronavirus and against any other human cold virus.
It's true, but of course that is completely misleading information, in theory this test would react against the old SARS coronavirus. But this has not happened in humans for 16 years. And theoretically, this test would also react against a whole range of bat coronaviruses, but they don't exist in humans either.
That last paragraph nails it. The common cold viruses are excluded, and the apparent a-specificity of the test, which is designed intentionally, can do no harm, for humans don't suffer from SARS-CoV or bat viruses at the moment.
Drosten also touches on the financial gains he might have received by distributing this test (remember that the Review Report stressed this "conflict of interest" aspect):
Christian Drosten: And the kind of technical validation is at such a high level, and we already published it in January, it was one of the first scientific publications ever about this new virus, that an incredibly large number of companies, not only in Germany, but in the all over the world, has started to transfer this test immediately into commercial validated test systems because of this good validation data. And at the same time, the World Health Organization immediately put this test protocol public on their side so that all public health agencies can use it.
And we immediately started distributing the essential material around the world. We started right away in mid-January, incidentally, free of charge, so we're involved in a research project that is being paid for by the European Union. They give us support for a personnel position that packs these packages that we send worldwide for RNA material. And the recipients on the other hand do not have to pay an allowance for the transport costs to us, but to this EU project (which, by the way, is coordinated at the University of Marseille).
Korinna Hennig: So, you don't make any money with it.
Christian Drosten: We don't earn a cent. On the contrary, we pay a lot on top of it. Fortunately, we are supported by public research funding from the European Union and, more recently, from the Bill Gates Foundation, Bill & Melinda Gates Foundation. But these are research funds that are specifically there for this purpose. We don't do any other research with it, but basically just pack packages with it. So we don't earn a penny, we pay more because we didn't have this research funding at the beginning. We just had to push it out of the postage because otherwise nobody would have done anything....
Korinna Hennig: So transparency not only in research, but also in the figures.
Here's a table summarizing the whole production and test flow of the Corman-Drosten PCR test:
|The raw material was gathered by China:
- Patient samples
- SARS-CoV-2 virus
- SARS-CoV-2 sequence (sent to Germany)
|Several tests were developed in Germany based on:
- SARS-CoV sequence
- Bat virus sequences
- SARS-CoV-2 sequence (received from China)
- Bat viruses
- SARS-CoV-2 (synthetic)
- Common cold viruses
- Influenza viruses
Etc. (20 known viruses in total)
To summarize: the Corman-Drosten test was based on a full SARS-CoV-2 genome received from Chinese researchers, who had assembled it within days. Given their expertise with SARS-CoV the Drosten team re-used an existing test protocol for this previous SARS virus, but also added specifics that would makes sure SARS-CoV-2 was matched. So there are generic and specific elements to this test. However, it is not so generic that viruses other than animal bat or SARS viruses are matched as well, and it can distinguish between SARS-CoV and SARS-CoV-2. As a proper test for this new virus should be able to do. And even if that SARS-CoV-2 element was skipped, the SARS-generic test would still make do because SARS-CoV is no longer circulating.
None of these basic facts were picked up by the two authors/contributors of the Review Report, who feature so erroneously as "courageous scientists" in the Naomi Wolf video interview. False heroism, if you ask me.
Prof. Andreas Beyer has offered a devastating critique of both the Review Report and the Addendum of the @BorgerPieter-Kämmerer team. He goes into great detail to expose the technical shortcomings of these texts and their clear agenda to discredit science. https://t.co/ltK7ZgaUpE pic.twitter.com/2aW70kHXa3— Frank Visser (@ReusVisser) May 3, 2021
HOW A CONSORTIUM OF NON-EXPERTS IS BUILT
The main objection of the Review Report main authors to the response from Eurosurveillance seemed to be that it did not address their many points of criticism. Well, it turns out that that work has already been done. On Researchgate I found a long article by Andreas Beyer, which reviewed the Corman-Drosten Review Report paper point-by-point. Beyer is currently a university professor in molecular biology at the Westfälische Hochschule in Recklinghausen, Germany. In this role he covers the fields of: standard methodology of molecular biology, DNA sequencing, PCR, biochemistry and computer-assisted sequence analysis. His competence by far exceeds those of most "top scientists in the life sciences" of the Consortium that published the Review Report.
As Beyer drily remarks when commenting on the "expertise" of the 22 authors/proofreaders of the Review Report: with few exceptions, none of them are molecular biologists with PCR-related experience. Now this has often been taken to involve unwarranted ad hominem attacks on the Consortium members, but that is most definitely not the case. Qualifications do matter here. It is highly relevant. With the exception of first author Pieter Borger and Kevin McKernan, none of these luminaries have evidenced any practical experience or theoretical expertise with this specialized field, not even the last author (who normally has a senior and supervising role in scientific articles), Ulrike Kämmerer, who specializes in cancer and nutrition!
Here's Beyer's breakdown of the Consortium members:
|CONSORTIUM MEMBERS||COMMENTS BEYER|
|Dr. Peter Borger (MSc, PhD), Molecular Genetics, W+W Research Associate, Lörrach, Germany (Researchgate)||"W+W" (for Wort und Wissen, "Word and Knowledge") is a creationist association. There are no laboratories and scientific research groups. And hence, there is also no "Research Associate".|
|Rajesh Kumar Malhotra (Artist Alias: Bobby Rajesh Malhotra), Former 3D Artist / Scientific Visualizations at CeMM - Center for Molecular Medicine of the Austrian Academy of Sciences (2019-2020), University for Applied Arts - Department for Digital Arts Vienna, Austria||A "3D-artist", seriously??|
|Dr. Michael Yeadon BSs(Hons) Biochem Tox U Surrey, PhD Pharmacology U Surrey. Managing Director, Yeadon Consulting Ltd, former Pfizer Chief Scientist, United Kingdom,||Former Pfizer employee (pharmacologist / toxicologist / manager) and anti-vaccationist / conspiracy theorist, not a molecular biologist|
|Dr. Clare Craig MA, (Cantab) BM, BCh (Oxon), FRCPath, United Kingdom||A pathologist is not a molecular biologist|
|Kevin McKernan, BS Emory University, Chief Scientific Officer, founder Medical Genomics, engineered the sequencing pipeline at WIBR/MIT for the Human Genome Project, Invented and developed the SOLiD sequencer, awarded patents related to PCR, DNA Isolation and Sequencing, USA (Researchgate)||Since decades McKernan is involved in science management and organisation, now he is chief sales officer and hence out of practical science since many years (Medical Genomics). ["When it comes to cannabis genetics and microbial detection, Medicinal Genomics is in a class of its own."]|
|Prof. dr. Klaus Steger, Department of Urology, Pediatric Urology and Andrology, Molecular Andrology, Biomedical Research Center of the Justus Liebig University, Giessen, Germany (Researchgate, auto-generated page)||An androgologist / urologist is not a molecular biologist|
|Dr. Paul McSheehy (BSc, PhD), Biochemist & Industry Pharmacologist, Loerrach, Germany||A biochemist / pharmacologist is not a molecular biologist|
|Dr. Lidiya Angelova, MSc in Biology, PhD in Microbiology, Former researcher at the National Institute of Allergy and Infectious Diseases (NIAID), Maryland, USA||We could not find any scientific publication of Dr. Angelova on Corona or on diagnostic PCR systems. Moreover, it seems as if she has a different job now and is no more active in science.|
|Dr. Fabio Franchi, Former Dirigente Medico (M.D) in an Infectious Disease Ward, specialized in “Infectious Diseases” and “Hygiene and Preventive Medicine”, Società Scientifica per il Principio di Precauzione (SSPP), Italy||A physician - microbiology, hygiene, infectiology - is not a molecular biologist. None of his three scientific, peer-reviewed papers has to do with PCR-systems.|
|Dr. med. Thomas Binder, Internist and Cardiologist (FMH), Switzerland||An internist / cardiologist is not a molecular biologist|
|Prof. Dr. med. Henrik Ullrich, specialist Diagnostic Radiology, Chief Medical Doctor at the Center for Radiology of Collm Oschatz-Hospital, Germany||A radiologist is not a molecular biologist. Besides this, he is the second author belonging to the pseudoscientific creationist organisation "Wort und Wissen" - he even is the chairman|
|Prof. Dr. Makoto Ohashi, Professor emeritus, PhD in Microbiology and Immunology, Tokushima University, Japan||Prof. Ohashi was a specialist for Epstein-Barr-Virus as well as viral induced lymphoma. He never was involved in Corona research or in development of diagnostic PCR systems.|
|Dr. Stefano Scoglio, B.Sc. Ph.D., Microbiologist, Nutritionist, Italy (Researchgate)||A microbiologist / nutritionist is not a molecular biologist|
|Dr. Marjolein Doesburg-van Kleffens (MSc, PhD), specialist in Laboratory Medicine (clinical chemistry), Maasziekenhuis Pantein, Beugen, the Netherlands (Researchgate)||A specialist in Laboratory Medicine is not a molecular biologist|
|Dr. Dorothea Gilbert (MSc, PhD), PhD Environmental Chemistry and Toxicology. DGI Consulting Services, Oslo, Norway (Researchgate)||An environmental chemist / toxicologist medicine is not a molecular biologist|
|Dr. Rainer J. Klement, PhD. Department of Radiation Oncology, Leopoldina Hospital Schweinfurt, Germany (Researchgate)||An oncologist is not a molecular biologist|
|Dr. Ruth Schruefer, PhD, human genetics/ immunology, Munich, Germany||PhD in immunology 2006. She seems to be out of science since then.|
|Dra. Berber W. Pieksma, General Practitioner, The Netherlands||General Practitioner? Seriously??|
|Dr. med. Jan Bonte (GJ), Consultant Neurologist, the Netherlands||An neurologist is not a molecular biologist|
|Dr. Bruno H. Dalle Carbonare (Molecular biologist), IP specialist, BDC Basel, Switzerland||This person is out of practical science since decades. He is involved in science management and organisation instead.|
|Dr. Kevin P. Corbett, MSc Nursing (Kings College London) PhD (London South Bank) Social Sciences (Science & Technology Studies) London, England, UK||Nursing and Social Sciences, seriously?|
Prof. Dr. Ulrike Kämmerer, specialist in Virology / Immunology / Human Biology / Cell
Biology, University Hospital Würzburg, Germany (Researchgate, auto-generated page)
Note: Prof. Dr. Ulrike Kämmerer is "Apl. Prof. Dr. rer. hum. biol.", this means "außerplanmäßiger Professor".(FV)
|Prof. Kämmerers expertise as given on her website at the Würzburg gynaecological hospital - where she is listed as research assistant - is human biology, virology, immunology and cell biology. However, the "research"-link on this site does not list virology. She is author of popular science books on cancer and nutrition. Her scientific work comprises quite a few papers on immunology, cancer, and reproduction biology. There is only a virological paper on enteroviruses (with her as coauthor) from 1998 and a paper on detection of picornavirus by PCR from 1995. There was a short e-mail exchange between her and me in December 2020. I asked her for her own experience and skills in qPCR. She refused to answer In other words: She neither is a virologist nor an expert in PCR. However, she distributes conspiracy theory, e.g. in "Die freie Welt" - an internet-newspaper of the right-wing populist party AfD, where she said "PCR-test is like reading the tea leaves".|
The main, or at least the last author of the Review Report paper, Ulrike Kämmerer, recently gave a rather curious justification of the doubtful expertise of the the Consortium members:
About the authors [of the Review Report] it was always said that we weren't active virologists who deal with SARS-CoV-2. PCR is a completely normal, mundane, routine method in every laboratory. I don't have to be a top virologist or anything on the contrary: the people who do their daily work with it, see where these technical flaws are much faster than someone who hovers somewhere above.
I don't have to be a bricklayer to see a wall is skewed. (p. 75)
Yet, how many of these 22 "experts" have this day-to-day experience with PCR testing? It is precisely those PCR specialists, like Andreas Beyer, Ian MacKay and others, who posses this expertise, and who have raised stern objections to this whole retraction project! It is precisely the bricklayers who have to be listened to here.
In fact, this whole notion of an "INTERNATIONAL CONSORTIUM OF SCIENTISTS IN LIFE SCIENCES (ICSLS)" which produced the Review Report is an inflated charade when it comes to relevant technical PCR expertise. In my opinion, Borger and McKernan could have co-authored it with more, not less credibility. And even then, as Beyer comments: Borger is currently a "research associate" at the Christian-creationist organization Wort und Wissen, which has no research division or laboratory, and McKernan is more of a sales manager for Medical Genomics (and specializing in the genomics of... cannabis. Hey, I am from Amsterdam).
Actually, in a recent interview the second author of the retraction paper, Bobby Malhotra, gives us some insight in how the "Consortium" came together:
On a day when I was very upset [after his contract was not renewed in Q4, 2020], I created a mega thread on Twitter with 124 tweets. In "Corona Ausschuss Nr. 22", Corona Committee No. 22, Prof. Dr. Ulrike Kämmerer had explained the problem of Drosten PCR tests. I linked this with my knowledge and tried to explain it for the beginner on Twittera mixture of corruption, bioinformatics and the PCR problem. This thread pretty much hit home.
This is how I came across Dr. Pieter Borger, who was already critical on ResearchGate at the same time. At some point he wrote to me, Mr. Malhotra, do you want to join me, I want to write a retraction for the Drosten paper. Then I had to open up to him that I am not a classical scientist at all, but a digital artist and self-taught bioinformatician. He was very surprised why I was able to talk to him at all about these issues. I then told him basically the same story I told you. After a meeting with Pieter Borger and Ulrike Kämmerer, everything was actually clear relatively quickly. We split up, asked our scientific circles and gathered 22 scientists... So we went on a search with Kevin McKernan. He's sort of our "Mister Technical Details," the technical details man, the "Mister PCR" of the scientific literature.
From him we know that this Charité [Corman-Drosten] primers & probes design is actually pretty garbage. We then put such technical details into the Addendum. For example, the RdRp gene has too low a sensitivity. The point is that you have to have a certain kind of sensitivity to be really sure that this result is true. It just isn't. (emphasis added)
1/124 This thread is going to examine & dissect the very specific behavioral-patterns, underlying market-architecture, fraudulent scientific methods, orthodox rituals of the vast pharma-wasp nest set around the queen wasp Christian Drosten (@c_drosten). #wild #ride #PCRGate pic.twitter.com/dVxoYJyzNf— Bobby Rajesh Malhotra ? (@Bobby_Network) October 27, 2020
As I wrote in Part 20, the reduced sensitivity of one part of the Corman-Drosten test is widely known, and hardly a reason to demand a retraction. I would say "the first pancake always fails" and the more pancakes we bake, the better they become.
A POINT-BY-POINT REBUTTAL OF BORGER ET AL.
Beyer calls the tone of the Review Report paper “presumptuous and insolent”. But on to the Review Report itself and its main points of criticism directed at the Corman-Drosten PCR protocol. I restrict myself to the SUMMARY CATALOGUE OF ERRORS FOUND IN THE PAPER of the Review Report, and will quote Beyer's comments in full:
|BORGER ET AL.||BEYER'S COMMENT|
|1. There exists no specified reason to use these extremely high concentrations of primers in this protocol. The described concentrations lead to increased nonspecific bindings and PCR product amplifications, making the test unsuitable as a specific diagnostic tool to identify the SARS-CoV-2 virus.||Everyone who is experienced in the design of such qPCR systems knows: The so-called standard concentrations only are guide values to start with. Appropriate concentrations have to be determined empirically. Hence it is failed to criticise a qPCR only on "theoretical" grounds without any laboratory test.|
|2. Six unspecified wobbly positions will introduce an enormous variability in the real world laboratory implementations of this test; the confusing nonspecific description in the Corman-Drosten paper is not suitable as a Standard Operational Protocol making the test unsuitable as a specific diagnostic tool to identify the SARS-CoV-2 virus.||
Here four severe errors of Borger et al have to be stated.
I: Viral genomes accumulate mutations, not equally distributed over the genome. For primers target positions have to be chosen that are as well conserved as possible, at the same time different to other groups. However, perfect targets in fact do not exist. Hence, positions with ambiguities, resulting in wobble-positions, have to be accepted. This is proper design rather than a design error.
II: Such wobble positions do surely not create "enormous variability". Primers with wobble positions from the start are synthesised and later used as defined mixtures.
III: For users, wobble positions do not cause confusion. Every professional PCR operator knows the respective code and knows how to handle it.
IV: PCR with wobble-primers will not automatically lose specificity. Specificity is determined during establishment and validation of the test. By the way, wobbleprimers are standard (not only) for HIV-diagnostics since decades.
|3. The test cannot discriminate between the whole virus and viral fragments. Therefore, the test cannot be used as a diagnostic for intact (infectious) viruses, making the test unsuitable as a specific diagnostic tool to identify the SARS-CoV-2 virus and make inferences about the presence of an infection.||
This argument is completely mistaken.
I: No test at all can discriminate between complete and incomplete / fragmented viral genomes.
II: The ability to such discrimination is unnecessary because each genome copyno matter if intact or damagedoriginates from a cell infected with an intact virus.
|4. A difference of 10° C with respect to the annealing temperature Tm for primer pair1 (RdRp_SARSr_F and RdRp_SARSr_R) also makes the test unsuitable as a specific diagnostic tool to identify the SARS-CoV-2 virus.||First, the authors confuse annealing temperature (which for both primers necessarily is identical) with melting temperature. Indeed, melting temperatures should be as similar as possible. But again: Everyone who has experience in PCR design knows that realityi.e. the sequence contextdictates what is feasible. Greater deviations of melting temperatures sometimes are inevitable. This is one more reason for exhaustive testing of a diagnostic qPCR system which has been done by Corman et al.|
|5. A severe error is the omission of a Ct value at which a sample is considered positive and negative. This Ct value is also not found in follow-up submissions making the test unsuitable as a specific diagnostic tool to identify the SARS-CoV-2 virus.||Again Borger et al. mix up technical terms. You never can predefine a Ct value, since Ct is measured during each qPCR run for each sample. Probably the threshold is meant instead. But this also does not make any sense, because the threshold is dependent from the detection system and the qPCR machine used. Obviously, - Borger et al. have no idea how to perform a qPCR test: Alongside with the samples, a dilution series is processed, serving as a standard to compare the samples with.|
|6. The PCR products have not been validated at the molecular level. This fact makes the protocol useless as a specific diagnostic tool to identify the SARS-CoV-2 virus.||Of course PCR fragments have been analysed by gel electrophoresis in the beginning! This is standard procedure! However, it needs not to be mentioned because specificity testing is more important. Moreover, the three independent oligonucleotides employed for TaqMan qPCR guarantee for high specificity. And the Drosten-Corman paper thoroughly documents validation of the qPCR system.|
|7. The PCR test contains neither a unique positive control to evaluate its specificity for SARS-CoV-2 nor a negative control to exclude the presence of other coronaviruses, making the test unsuitable as a specific diagnostic tool to identify the SARS-CoV-2 virus.||The opposite is true. The Corman-Drosten paper describes some positive and negative controls as well as thorough validation. Precise setup of the testincluding controlsis up to each individual user. And the user also has to define his own SOP.|
|8. The test design in the Corman-Drosten paper is so vague and flawed that one can go in dozens of different directions; nothing is standardized and there is no SOP. This highly questions the scientific validity of the test and makes it unsuitable as a specific diagnostic tool to identify the SARS-CoV-2 virus.||Half of the claim is untrue, the other half is irrelevant. Indeed, the Corman-Drosten paper specifies all relevant information: Primer and probe sequences, concentrations, PCR conditions. On the other hand, formulation of the SOP is up to the user and not to the publisher of a test.|
|9. Most likely, the Corman-Drosten paper was not peer-reviewed making the test unsuitable as a specific diagnostic tool to identify the SARS-CoV-2 virus.||Indeed, peer reviews usually take days or even weeksEurosurveillance, however, is known for high speed. Sometimesespecially when authors and journal agree that publication is urgentarrangements are made: Either reviewers may stand by the very hour the paper is filed. Or the paper is filed and reviewed bit by bit. In such a case the noted day of submission is the day when the last part or the last data were filed. This may well be the day of approval. By the wayin the case of the CormanDrosten publication, the paper was available to reviewers one week before on a preprint server.|
|10. We find severe conflicts of interest for at least four authors, in addition to the fact that two of the authors of the Corman-Drosten paper (Christian Drosten and Chantal Reusken) are members of the editorial board of Eurosurveillance. A conflict of interest was added on July 29 2020 (Olfert Landt is CEO of TIB-Molbiol; Marco Kaiser is senior researcher at GenExpress and serves as scientific advisor for TIB-Molbiol), that was not declared in the original version (and still is missing in the PubMed version); TIB-Molbiol is the company which was "the first" to produce PCR kits (Light Mix) based on the protocol published in the Corman-Drosten manuscript, and according to their own words, they distributed these PCR-test kits before the publication was even submitted; further, Victor Corman & Christian Drosten failed to mention their second affiliation: the commercial test laboratory "Labor Berlin". Both are responsible for the virus diagnostics there and the company operates in the realm of real time PCR-testing.||Conflicts of interest only exist if the respective coauthors had an advantage from the publication, e.g. by marketing and distribution of kits or by gaining market share. However, the described qPCR system is freely available and applicable for everyone and reagents can be purchased from any distributor, Hence it is hard to interpret this claim of Borger et al. different than vicious slander.|
There are other pertinent points raised in the Beyer paper, but I leave it to you to pursue this further, if that's your interest. But as you can see from the above, there is, again, a world of difference between the belligerent and accusative mood of the Corman-Drosten Review Report and the informed opinions of experienced scientists, who know that what works in theory doesn't always do so in practice. As we say in Dutch: "The best helmsmen stand on shore" (which seems to be equivalent to: "Bachelors' wives and maidens' children are well taught", source: Heywood, 1546).
Beyer is even more blunt in his final conclusions:
The authors claim to be able to refute the Drosten paper on scientific grounds.
However, if you want to criticise a scientific paper, you should do this in the form of another scientific paper, published in a scientific journal with peer review (!) and not in the form of a private www-domain. This fact alone already renders this "peer review" highly dubious. Hence, as long as the text by Borger et al. has not been checked and accepted by a scientific journal (which will not happen due to its blatant errors) it is nothing than expression of opinion of some laypeople. Second, the fact that the authors did not perform a single experiment but argue alone on basis of "theoretical considerations" is more than questionable. Third, the authors have no expertise in analytic / diagnostic PCR systems. There is only a single virologist among themProf. Ohashi. And he has no experience in corona research. The ignorance of the authors can easily be recognised by the flawed critique they utter. Some of the mistakes they make even are beginner's mistakes. It is nothing but imprudence to criticise on such weak grounds a peer-reviewed paper from an internationally well-respected scientific group. Fourth, the respective qPCR-system has now been used worldwide since several months. If it was faulty, if it had any of the claimed impairments it would have been criticised long ago. In contrarymeanwhile there have quite a few comparisons of existing PCR assays been published (z. B. Afzal 2020, Matheeussen et al. 2020, Nalla et al. 2020, Vogels et al. 2020). No one has found severe drawbacks in the Corman-Drosten assay (just a less sensitive RdRp-assay in comparison to others, that was all). Fifth, it can easily be shown that all critical points submitted by the authors implode under closer inspection. However, quite a bit technical knowledgenot available for laypeopleis necessary to see through this flawed argumentation. And so, this "paper" already has become famous among conspiracy theory proponents, anti-vaccationists and creationists: Audacter calumniaresemper aliquid haeret... [slander boldly, something always sticks]. Last, Borger et al. were given the chance to comment on my arguments and to defend themselves. They kept silent.[See their response in Appendix 2]
In all, this "peer review" is at best pseudoscienceif not conspiracy theory and slander.
In the meantime, Bobby Malhotra, who we met in the video and who features as the second author of the Review Reportand is for sure the most vindicative and the least qualified of all, being a 3D artisthas already announced an official reply to Beyer's paper, again losing all sense of perspective and proportion (there we have the ad hominems again!).
We will release a refute of this utter nonsense soon. TL:DR: utter nonsense, circular reasoning, and he gets all the facts false, even our biographies. We are not in a hurry to refute his points, they are full of ad hominems, etc.— Bobby Rajesh Malhotra ? (@Bobby_Network) February 6, 2021
But we will refute them nevertheless soon.
So it's "totally wrong" vs. "utter nonsense"? Not a good climate for productive debate. Two paradigms or false equivalence? "Free and reliable science" or "political and pseudoscience" (as Pieter Borger frames it)? I would not put my bet on the Consortium. They will predictably complain, "Yes, but what about our Addendum?" But if the theoretical foundations of their Review Report are so problematic, why bother?
Appendix 1 : Dan Wilson's Debunk of the Retraction Request
Dan Wilson, a PhD scientist in molecular and structural biology, has a YouTube channel "Debunk the Funk with Dr. Wilson", on which I found this debunk of the Borger et al. paper. He has also debunked Kaufman, Cowan, the Chromosome 8 canard, Michael Yeadon, the lab orgin theory, and many other topics covered in THE CORONA CONSPIRACY, so he's definitely a kindred spirit.
|CLAIMS BORGER ET AL.||COMMENTS WILSON|
|The PCR test is based on a theoretical model of the virus||The PCR test is based on the full genome of the virus|
|It is impossible to make a valid PCR test without a virus isolate||This is just wrong. Though not ideal, it is entirely possible|
|Primer temperatures used in this PCR protocol are too high||It is well within the range of what people normally use.|
|Wobbly base pairs in primers and probes cause confusion||Wobbly base pairs were used for unconserved regions|
|The test detects fragments, not the full genome of the virus||PCR tests only detect fragments, by design|
|The number of amplification cycles used is too high||The positives show up in real time, irrespective of the maximum|
|Setting a high number of amplification numbers is useless||It can still be useful to find pre- or post-sympomatic cases|
|Biological validations a lacking in this paper||True, but it doesn't make the paper invalid|
|There was no peer review done on this paper||The peer review for this paper was fast-tracked|
|Some authors of this paper are also editors of the journal||This happens sometimes, but it doesn't make the paper invalid|
|This test produces too many or only false positivies||Where are all these false positives, seriously?|
He concludes: why do we find positives only in countries that are still impacted by the virus, with many hospitalized cases and deaths, and not in countries were they have seemed to have beaten the virus (even though wide-scale testing has continued)?
Ultimately this critique [by Borger et al.] is nothing more than conspiracy theories with an illusion of expertise behind it. It offers really no helpful critiques, nothing of scientific substance to this conversation and only serves to galvanize the COVID-denying movement.
Ironically the more people deny COVID the more dangerous the virus gets.
Another astute observation by Wilson: "this Corman-Drosten paper reflects both the good and the bad parts of science during this pandemic." He recommends reading Ed Young's piece for The Atlantic: "How Science Beat the Virus, and what it lost in the process". From which:
[T]he COVID-19 pivot has also revealed the all-too-human frailties of the scientific enterprise. Flawed research made the pandemic more confusing, influencing misguided policies. Clinicians wasted millions of dollars on trials that were so sloppy as to be pointless. Overconfident poseurs published misleading work on topics in which they had no expertise. Racial and gender inequalities in the scientific field widened.
Appendix 2 : A "Refutation of Andreas Beyer's criticism"
A 22 page "refutation" of Beyer's paper has been published on the ICSLS website in mid February 2021. Its main objection was: Beyer has not read our Addendum. Well, the Review Report should stand on its own merit, and should not need any Addendum to strengthen its case, in my opinion, Beyer finds flaws in the Report which need to be addressed. (Another matter is if the Addendum really provides empirical support for the thesis of the Review Report, or if it presents a more nuanced picture).
Furthermore, it is argued that Beyer hasn't published much scientific articles in the relevant field, so his "ad hominem" attack on the Consortium members is misplaced. However, Beyer seems to have a lot of hands on experience when it comes to PCR, contracy to most of the Consortium members. (As an aside, Beyer has also written about creationism and evolution, so that overlaps with Borger's fields of interest). On Researchgate the two gentlemen corresponded briefly (11 February 2021). Borger asked "Why is it not allowed to express scientific concerns when a paper contains flaws?". Beyer's reply: "Good question! Exactly this is what I do with the Borger text."
One interesting point raised by Borger et al., is that, in their opinion (also voiced by Ulrike Kämmerer), there was no need for a Drosten test protocol at all, because the Chinese researchers, who where the first to produce the full genome of SARS-CoV-2, already had a test.
There was no pandemic situation at the given timeframe but an outbreak of pneumonia in China with 6 deaths in Hubei province, as stated and referenced in our main review report. The chinese scientists had everything of particular interest already available [5 articles by Chinese researchers are mentioned]:
There was no actual need for any hurry in Europe, the authors just could have asked the Chinese colleagues about their findings and the corresponding raw data. Why did they assume the chinese scientists would need any help with a PCR-protocol design out of Europe? While Corman et al. designed their flawed PCR protocol-design, the chinese CDC had already sequenced the alleged agent, generated the starting-point for a phylogenetic tree and established a diagnostic RT-qPCR in the first week of January. (p. 8)
- the alleged isolated virus (from primary patient samples),
- the sequences,
- their own PCR protocol-design.
Of course, the quality of the Chinese tests is relevant here, and not discussed in this "Refutation". Ironically, in conspiracy circles China is often portrayed as the Evil Empire, so at least this is a nice admission to have on record. (Notice the word "alleged", which leaves room for speculation about the real cause of COVID-19). And in retrospect, blaming the Drosten team for assuming a worldwide pandemic sounds a bit odd. They were very prescient indeed, given the two millons deaths a year later.
Though these Borger vs. Beyer exchanges still look like trench warfare, with no party conceding any ground, it could be the start of a more meaningful discussion. Beyer plans to address the Addendum when his busy teaching work schedule permits.
NOTE: This reply by Andreas Beyer has been written and can be found in Part 33, including subsequent responses from Borger.
Appendix 3: Stephen Bustin On the nature and value of PCR testing
In a recent article (Feb. 2021), PCR authority Stephen Bustin (who was one of the reviewers consulted by Eurosurveillance in its evaluation of the Review Report and is the author of the authoritative handbook A-Z of Quantitative PCR, 910 pages, 2004), reflects on the nature and value of PCR testing. Here's the abstract:
Although molecular testing, and RT-qPCR in particular, has been an indispensable component in the scientific armoury targeting SARS-CoV-2, there are numerous falsehoods, misconceptions, assumptions and exaggerated expectations with regards to capability, performance and usefulness of the technology. It is essential that the true strengths and limitations, although publicised for at least twenty years, are restated in the context of the current COVID-19 epidemic. The main objective of this commentary is to address and help stop the unfounded and debilitating speculation surrounding its use.
He also mentions the Corman-Drosten test in passing, and gives the following balanced judgement. Though the feedback given to Eurosurveillance about the Corman-Drosten Review Report has been not made public, I am sure Bustin had it in mind when he wrote these lines:
This test, targeting three SARS-CoV-2 genes, was not ideal as primer and probes were not optimised and it was not as sensitive as later iterations. However, the assay worked and was specific and demonstrated astounding sagacity and selflessness by the scientists involved, as well as the remarkable speed with which PCR-based tests can be developed and put into practice. Since then a large number of additional tests and improved protocols have been created by individual research groups and companies in many countries targeting a range of SARS-CoV-2 specific sequences, some detecting multiple targets, others able to detect its presence in crude samples, and yet others generating results in minutes. This means that we have a whole panoply of kits available using different reverse transcriptases, Taq polymerases and buffer combinations, all of which can detect SARS-CoV-2. (emphasis added)
Remarkably, where the authors of the Corman-Drosten Review Report framed the Corman-Drosten test negatively within a negative approach to testing for COVID-19, Bustin does the exact opposite. And even though he acknowledges some shortcomings regarding this early RT-qPCR test, he puts it firmly in the context of a strong pro-testing approach aiming at continuous improvement of the various ways of testing at our disposal. No need for any hysterical conclusions.
Appendix 4: Andreas Beyer's reply to Borger et al.'s Report and Addendum
In a 8500 words essay, prof. Andreas Beyer has offered a devastating critique of both the Review Report and the Addendum of the Borger-Kämmerer team. He goes into great detail to expose the shortcomings of these texts and their clear agenda to discredit science:
Here, a general problem of the Borger-Kämmerer alliance becomes obvious: It is not enoughand it is not scienceto put some sequence data into some analysis software and enjoy the output. One has to understand and interpret the output and this requires well-funded theoretical knowledge and practical skillsobviously not available in the Borger-Kämmerer alliance...
To get to the point: Indeed, the Corman-Drosten qPCR system "version 1.0" was "immature" which no one denies. However, already "version 2.0" was fully functional long before the Borger-Kämmerer text was launched (!)... Various tests have shown the weak points in "version 1,0", but no one drew the conclusion that it is completely unsuitable as claimed by the Borger-Kämmerer group...
Another critical point is the way the Borger-Kämmerer group utilises scientific literature. They pick out whatever seemingly supports their argumentation and ignore the rest. And in doing so citations are sometimes presented out of their context.
And he concludes:
The Borger-Kämmerer text is pseudoscience, it is full of misconceptions, errors and flaws. Therefore it is ignored by experts for good reason. The impact it had in public consciousness, however, is fatal. Borger reported on Twitter more than 30 Million views of his "Report" (March 2021) [now 50 million]. Hence I ask all colleagues please to spread this essay for at least a little bit of counterbalance.
The authors of the ICSLS Consortium have specifically expressed their wish for scientists to reflect on their studies of the Corman-Drosten test. Methinks they can't wish for a better treatment than a critical review.
I leave it to you to dive into the dirty details by reading the essay on ResearchGate.
 Victor M Corman et. al., "Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR", PMC, www.ncbi.nlm.nih.gov, (Euro Surveillance, 2020 Jan 23).
 Pieter Borger et. al., "Review report Corman-Drosten et al. Eurosurveillance 2020", External peer review of the RTPCR test to detect SARS-CoV-2 reveals 10 major scientific flaws at the molecular and methodological level: consequences for false positive results, November 27, 2020.
 Naomi Wolf, "PCR Lab Visit: Whistleblowers Kevin McKernan, Bobby Malhotra Explain Why COVID Tests are 'Garbage'", YouTube, 24 Jan 2021.
[3a] Coronaviridae Study Group of the International Committee on Taxonomy of Viruses, "The species Severe acute respiratory syndrome-related coronavirus: classifying 2019-nCoV and naming it SARS-CoV-2", Nat Microbiol. 2020; 5(4): 536-544.
 Korinna Hennig, "Coronavirus Update, Folge 16", NDR, March 18, 2020.
 Andreas Beyer, "Pseudowissenschaftliche Kritik an einem von der Berliner Charité veröffentlichten Coronavirus (SARS-CoV-2)-Test, publiziert von Peter Borger und Koautoren / Pseudoscientific criticism on a Coronavirus (SARS-CoV-2) test published by Peter Borger and coauthors-PCR-Borger-Report", January 2021, www.researchgate.net (PDF). A nice summary in German can be found here: Andreas Beyer, "COVID-19 und Kreationismus: Widerlegen Borger et al. die Drosten-PCR? Die unheilige Allianz der Verschwörungstheoretiker", www.ag-evolutionsbiologie.net, January 23, 2021.
 "Illa", Das PCR-Desaster, Genese und Evolution des "Drosten-Tests", Verlag Thomas Kubo, 2021 (PDF). Kämmerer's quote comes from a partial transcript from: 38. Sitzung zum Thema "Angriff auf Mensch und Gesellschaft" (corona-ausschuss.de, February 5, 2021), which is inluded in this book.
 Aya Velázquez, "We are in the middle of an information war", medium.com, Febuary 3, 2021.
 Ed Young, "How Science Beat the Virus, and what it lost in the process", The Atlantic, December 14, 2020.
 "Refutation of Andreas Beyer's critisism", cormandrostenreview.com, no date (mid February 2021). The page is now password protected and apparently still work in progress (Version 2). Version 1 is archived here: https://archive.is/UevlP
 Stephen Bustin et al., "COVID-19 and Diagnostic Testing for SARS-CoV-2 by RT-qPCR Facts and Fallacies", International Journal of Molecular Sciences, 28 February 2021 (PDF).
 Andreas Beyer, "Borger & Kämmerer, Corona & qPCR, Pseudoscience & Conspiracy Theory Revisited - an Analytical Essay", ResearchGate, May 2021.
83 Vaccine Myths from docbastard.net